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Conclusion The need for further research to develop better methods and research based policies for safe cigarette smoking and workable cessation strategies must be a priority in order to deal with the tobacco epidemic

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Systems toxicology analyses detected biological effects of nicotine exposure, which included up-regulation of the xenobiotic-metabolizing enzymes CYP1A1 and FMO3 in the lung and metabolic effects, likely interlinked with a generalized stress response to nicotine present in the exposure aerosols [34]

acknowledges support from the National Heart Foundation of Australia (107153)